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l4440 control empty vector  (Addgene inc)


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    Addgene inc l4440 control empty vector
    L4440 Control Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l4440 control empty vector/product/Addgene inc
    Average 95 stars, based on 249 article reviews
    l4440 control empty vector - by Bioz Stars, 2026-03
    95/100 stars

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    ( A ) Histogram of primary GABA-specific RNAi screen. 79 RNAi clones (green bars) were selected for retesting with aldicarb hypersensitivity above cutoff (dotted line) of two standard deviations above the mean. μ = 14.40%, σ = 12.15%, cutoff = 38.70%. ( B ) Aldicarb sensitivity of controls from primary screen. Negative control <t>(L4440,</t> blue box) fed worms are significantly less sensitive to aldicarb than positive control ( unc-25 , green box) fed worms. N = 42 for each condition. Whiskers are min to max values. p<0.0001. ( C ) Hits from secondary screen. RNAi against 48 genes causes hypersensitivity to aldicarb above primary RNAi screen cutoff in at least 3 independent trials of ∼25 worms each. p<0.0001 for all clones compared to control RNAi.
    L4440 Empty Vector Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Developmental cell

    Article Title: Comprehensive endogenous tagging of basement membrane components reveals dynamic movement within the matrix scaffolding

    doi: 10.1016/j.devcel.2020.05.022

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: 2017 NK2318 ten-1::LL::mNG CRISPR knock-in; qy56 This study NK2502 ddr-1::LL::mNG CRISPR knock-in; qy43 This study NK2456 ddr-2::LL::mNG CRISPR knock-in; qy44 This study NK2457 ptp-3::LL::mNG CRISPR knock-in; qy47 This study NK2477 sdn-1::LL::mNG CRISPR knock-in; qy29 This study NK2413 dgn-2::LL::mNG CRISPR knock-in; qy 82 This study NK2587 gon-1::LL::mNG CRISPR knock-in; qy45 This study NK2590 mig-17::mNG::3xFlag; shc19 Ji et al. 2019 FDU1506 Oligonucleotides Table S2 Recombinant DNA pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA) Addgene 47550 mNG-C1^SEC^linker This study mNG-SEC-LL mNG-C1^SEC^bothlink This study mNG-SEC-bothlink mEos2-C1^SEC This study mEos2-SEC L4440 RNAi empty vector control Addgene 1654 mig-6 RNAi This study / Ahringer mig-6 RNAi Software and Algorithms Fiji Schindelin et al., 2012 Image J 2.0.0-rc-69/1.52p, http://fiji.sc/ Photoshop Adobe Version CC 2018 Imaris Bitplane Version 9 Illustrator Adobe Version CC 2018 R GNU Project Open Source μManager Edelstein et al., 2010 Version 1.4, https://micromanager.org/ iLas Biovision V.1.2.3 MetaMorph Molecular Devices 7.10.3.279 Excel Microsoft Version 15.39 GraphPad Prism GraphPad Version 7 Open in a separate window KEY RESOURCES TABLE In vivo visualization of 29 basement membrane (BM) proteins fused with mNeonGreen Embryonic BMs share a common template but diversify greatly during development Stable BM proteins form a scaffold that supports movement of dynamic BM components Papilin promotes local BM collagen removal by limiting GON-1 protease localization

    Techniques: Virus, Recombinant, Mutagenesis, CRISPR, Knock-In, Clinical Proteomics, Membrane, Plasmid Preparation, Control, Software

    KEY RESOURCES TABLE

    Journal: Developmental cell

    Article Title: Comprehensive endogenous tagging of basement membrane components reveals dynamic movement within the matrix scaffolding

    doi: 10.1016/j.devcel.2020.05.022

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: L4440 RNAi empty vector control , Addgene , 1654.

    Techniques: Virus, Recombinant, Mutagenesis, CRISPR, Knock-In, Clinical Proteomics, Membrane, Plasmid Preparation, Control, Software

    ( A ) Histogram of primary GABA-specific RNAi screen. 79 RNAi clones (green bars) were selected for retesting with aldicarb hypersensitivity above cutoff (dotted line) of two standard deviations above the mean. μ = 14.40%, σ = 12.15%, cutoff = 38.70%. ( B ) Aldicarb sensitivity of controls from primary screen. Negative control (L4440, blue box) fed worms are significantly less sensitive to aldicarb than positive control ( unc-25 , green box) fed worms. N = 42 for each condition. Whiskers are min to max values. p<0.0001. ( C ) Hits from secondary screen. RNAi against 48 genes causes hypersensitivity to aldicarb above primary RNAi screen cutoff in at least 3 independent trials of ∼25 worms each. p<0.0001 for all clones compared to control RNAi.

    Journal: PLoS Genetics

    Article Title: Neuron-Specific Feeding RNAi in C. elegans and Its Use in a Screen for Essential Genes Required for GABA Neuron Function

    doi: 10.1371/journal.pgen.1003921

    Figure Lengend Snippet: ( A ) Histogram of primary GABA-specific RNAi screen. 79 RNAi clones (green bars) were selected for retesting with aldicarb hypersensitivity above cutoff (dotted line) of two standard deviations above the mean. μ = 14.40%, σ = 12.15%, cutoff = 38.70%. ( B ) Aldicarb sensitivity of controls from primary screen. Negative control (L4440, blue box) fed worms are significantly less sensitive to aldicarb than positive control ( unc-25 , green box) fed worms. N = 42 for each condition. Whiskers are min to max values. p<0.0001. ( C ) Hits from secondary screen. RNAi against 48 genes causes hypersensitivity to aldicarb above primary RNAi screen cutoff in at least 3 independent trials of ∼25 worms each. p<0.0001 for all clones compared to control RNAi.

    Article Snippet: The following RNAi clones were used: L4440 empty vector control (clone pPD129.36, Fire Kit, Addgene), GFP (clone pPD128.110, Fire Kit, Addgene), snb-1 (clone T10H9.4, Ahringer Library , unc-13 (clone ZK524.2, Ahringer Library), cat-2 (clone B0432.5, Ahringer Library), dop-3 (clone T14E8.3, Ahringer Library), eat-4 (clone ZK512.6, Ahringer Library), glr-1 (clone C06E1.4, Ahringer Library), unc-25 (clone Y37D8A.23, Vidal ORFeome Library , unc-49 (clone T21C12.1, Vidal ORFeome Library).

    Techniques: Clone Assay, Negative Control, Positive Control, Control

    Essential gene RNAi clones that affect GABA neuron function.

    Journal: PLoS Genetics

    Article Title: Neuron-Specific Feeding RNAi in C. elegans and Its Use in a Screen for Essential Genes Required for GABA Neuron Function

    doi: 10.1371/journal.pgen.1003921

    Figure Lengend Snippet: Essential gene RNAi clones that affect GABA neuron function.

    Article Snippet: The following RNAi clones were used: L4440 empty vector control (clone pPD129.36, Fire Kit, Addgene), GFP (clone pPD128.110, Fire Kit, Addgene), snb-1 (clone T10H9.4, Ahringer Library , unc-13 (clone ZK524.2, Ahringer Library), cat-2 (clone B0432.5, Ahringer Library), dop-3 (clone T14E8.3, Ahringer Library), eat-4 (clone ZK512.6, Ahringer Library), glr-1 (clone C06E1.4, Ahringer Library), unc-25 (clone Y37D8A.23, Vidal ORFeome Library , unc-49 (clone T21C12.1, Vidal ORFeome Library).

    Techniques: Clone Assay, Positive Control, Binding Assay, Plasmid Preparation, Control